We invite original research submissions on Software Engineering and Industrial IoT in the Second Workshop on Software Engineering in Industry 4.0 Ecosystems, co-located with the 8th International Conference on Emerging Data and Industry (EDI40) in Patras, Greece! This year's theme focuses on maintaining Software and Data in interconnected Industry 4.0 environments. The workshop will explore the long-term stability, security, and sustainability of software systems and data assets amidst evolving technologies such as firmware, operating systems, libraries, programming languages, databases, and AI-driven processes.