The IWBBIO 2025 (12th International Work-Conference on Bioinformatics and Biomedical Engineering) seeks to continue the good synergy obtained in the previous edition, providing a discussion forum for scientists, engineers, educators and students about the latest ideas and realizations in the foundations, theory, models and applications for interdisciplinary and multidisciplinary research encompassing disciplines of computer science, mathematics, statistics, biology, bioinformatics, and biomedicine.